Understanding gene regulation and lactose intolerance
This unit will introduce students to lactose intolerance. Students will develop questions about a case of a girl with symptoms of stomach pains, gas, and diarrhea. Throughout the unit, students will analyze pieces of evidence to answer their questions and eventually come to understand the mechanism of lactose intolerance.
Looking into Lactase is an activity developed by Learning Undefeated for the exploration of enzymatic activity by investigating the mechanism behind lactose intolerance.
Lactase d eficiency, also known as lactose intolerance, is a condition caused by an absence of the enzyme lactase, a digestive enzyme found in the human body. Since the human body can’t absorb lactose it needs to be broken down by lactase into its two monosaccharide products, glucose and galactose. Like most other enzymes, lactase is specific to just one substrate, in this case, the sugar lactose. Those who are lactose intolerant seek treatment through medications that contain lactase as an active ingredient.
Enzymes are proteins that catalyze chemical reactions by lowering the reaction’s activation energy. Every enzyme has an active site that binds to another molecule called the substrate. Once bound to the substrate, an enzyme can catalyze a reaction up to 10 billion times faster than the comparable, non-catalyzed reaction. Enzymes are not consumed in the reaction and can bind to an infinite amount of substrates.
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Learning Objectives
Carbohydrates (monosaccharides and polysaccharides)
Mutations in a gene can change gene function
Mutations in regulatory regions can change gene expression
Enzyme substrate relationship
Monosaccharides are building blocks of polysaccharides
Enzymes can break down polymers into monomers
Enzymes are specific to a substrate
Gene expression is regulated
Analyze data to determine if Maria had an allergic reaction
Analyze data to determine milk types based on glucose data and response to specific enzymes
Analyze RT-PCR data to determine the expression of LCT in Maria
Collect data about lactase’s activity in different environments to determine where in the body lactase is usually expressed.
Standards Alignments
HS-LS3-1: Ask questions to clarify relationships about the role of DNA and chromosomes in coding the instructions of characteristic traits passed from parents to offspring.
B.6D: recognize that gene expression is a regulated process
B.6E: identify and illustrate changes in DNA and evaluate the significance of these changes
Unit Plan
Students should be introduced to the story of Maria, a girl who recently started having symptoms of diarrhea, gas, and stomach pains. They will also be given the food she consumed that day in addition to her family’s medical history. After reading Maria’s story, students are encouraged to ask questions about what is causing Maria’s symptoms.
Did she eat anything else that could have caused this?
Could she be allergic to something in the milk or the other foods?
Was the milk expired?
What kind of milk was it?
When does her mother get these symptoms?
Did she get a disease from her mom’s side of the family?
Activities to Gather Evidence
Allergic Response Data
Students will be given blood count data for Maria and two control groups. Students will be asked to determine the differences between the normal and allergic samples and draw conclusions about Maria’s situation
Students will analyze the differences between soy and cow milk and will use a computer model to compare the molecular structures of sucrose and lactose.
Students analyze data about glucose concentration of milk samples and enzyme use in order to determine which sample contained which milk type. Students learn about enzymes and their functions.
Students learn that Maria has a mutation in a gene upstream of LCT. Students learn about gene expression in eukaryotes and how a mutation in a regulatory element can change a gene’s expression.
Students use a simulation to see what conditions (pH and temperature) allow for the highest enzymatic rate. Students will compare this information to data about temperature and pH throughout the human body to determine where lactase expression is usually turned on.
Students investigate the enzyme lactase and use the enzyme to help them identify the sugars that are present in three milk samples: rice milk, soy milk, and cow’s milk.
Students explore the use of DNA profiling and gel electrophoresis, using restriction enzymes and agarose gel electrophoresis to create a genetic profile.